Addgene

We narrowed to 32,562 results for: IND

Showing: 781 - 800 of 32562 results

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p5xUAS-Flag-NPM1c

Plasmid

#131823

Purpose

4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKan-PRFA1-9myc-AID*(N)

Plasmid

#99528

Purpose

N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

RFA1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)-9myc

Plasmid

#99526

Purpose

N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB cdc42 QL

Plasmid

#61636

Purpose

mammalian expression of cdc42 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Cdc42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

FLAG and LacR

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB rhoA QL

Plasmid

#61632

Purpose

mammalian expression of rhoA constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

rhoA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Q63L, constitutively active

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Use

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
-227 hTF (mutant NF-kB)

Plasmid

#15443

Depositor

Nigel Mackman

Article

Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

NF-kappaB binding domain mutated

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
p258 pSP72 hAR-LBD

Plasmid

#12492

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Use

Tags

Expression

Bacterial

Mutation

AR ligand binding domain

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCLXSN(GFP)-HA-Tbkbp1

Plasmid

#125177

Purpose

Retroviral expression of mouse Tbkbp1

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)

Plasmid

#99513

Purpose

Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-114) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

Expression

Mutation

internal inversion of nucleotides 265-312 of the …

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Use

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
LT3GEPIR-WT1shRNA

Plasmid

#220560

Purpose

To inducibly knockdown WT1 or EWSR1::WT1 expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

WT1 shRNA (WT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

Plasmid

#205603

Purpose

Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinWT-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

Plasmid

#205604

Purpose

Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinΔGTP-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A, N229A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5mutCBS

Plasmid

#131835

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Use

Tags

Expression

Mammalian

Mutation

partial intron 4 full exon 5 and partial intron 5…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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