We narrowed to 2,452 results for: LRE

1. pCAT009

   Plasmid

   #171635

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSyn-EGFP

   Plasmid

   #114213

   Purpose

   AAV vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-PD-1-SNAP

   Plasmid

   #223576

   Purpose

   Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Dyskerin

   Plasmid

   #207577

   Purpose

   N-terminal mCherry-tag using SalI/EcoRI

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

   Plasmid

   #62904

   Purpose

   Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4DBD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

   Plasmid

   #62949

   Purpose

   Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-LexA::QFAD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

   Plasmid

   #86827

   Purpose

   Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   FLAG-NLS-MS2-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

9. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. HaloTag-ER-3

    Plasmid

    #186960

    Purpose

    ER lumen localizaing HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CALR (CALR Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV MCL1 Puro

    Plasmid

    #140746

    Purpose

    Expresses MCL1 in mammalian cells

    Depositor

    Scott Dixon

    Article

    Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

    Insert

    MCL1 (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

    Plasmid

    #213398

    Purpose

    transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

    Depositor

    Katrin Willig

    Article

    Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

    Insert

    FingR for endogenous PSD95, transcriptinally regulated
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFR-FR

    Plasmid

    #179263

    Purpose

    Human EGFR C-terminally labeled with FusionRed

    Depositor

    Jared Toettcher

    Article

    Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK1

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. L1-neo-TET

    Plasmid

    #51284

    Purpose

    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    L1RE1 (L1RE1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19_CRISPR_DpmrA

    Plasmid

    #160903

    Purpose

    CRISPR_Cas9, lambda red recombineering

    Depositor

    Anne-Catrin Uhlemann

    Article

    McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

    Inserts

    Cas9
    Lambda Red Recombineering Genes
    Zeocin Resistance Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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