We narrowed to 6,025 results for: plasmid dna

1. pcDNA3_QLuc

   Plasmid

   #183260

   Purpose

   Express QLuc in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   QLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GtCCR2_EYFP_pcDNA3.1

   Plasmid

   #159121

   Purpose

   This plasmid encodes GtCCR2 rhodopsin domain

   Depositor

   John Spudich

   Article

   Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

   Insert

   GtCCR2
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GtCCR1_EYFP_pcDNA3.1

   Plasmid

   #159120

   Purpose

   This plasmid encodes GtCCR1 rhodopsin domain

   Depositor

   John Spudich

   Article

   Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

   Insert

   GtCCR1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MvChR1_EYFP_pcDNA3.1

   Plasmid

   #159119

   Purpose

   This plasmid encodes MvChR1 rhodopsin domain

   Depositor

   John Spudich

   Article

   Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

   Insert

   MvChR1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_SARS2_XBB

   Plasmid

   #194494

   Purpose

   expressing SARS-CoV-2 XBB spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (XBB) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

   Promoter

   CMV

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pELF2.1.0-gDNA

   Plasmid

   #132454

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ELF2 (ELF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRCA2.1.0-gDNA

   Plasmid

   #132448

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLF6.1.0-gDNA

   Plasmid

   #132435

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   KLF6 (KLF6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.3_CoV2_B.1.1.7

   Plasmid

   #170451

   Purpose

   Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Yuan et al Science. 2021 Aug 13;373(6556):818-823.

   Insert

   SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid

    #157716

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA3.1-SARS-CoV-2-S-ectodomain

    Plasmid

    #235984

    Purpose

    Internally tagged SARS-CoV-2 spike ecto-domain

    Depositor

    Syed Saif Hasan

    Article

    Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

    Insert

    Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    8xHis and Twin-Strep tag and internal Twin-Strep …

    Expression

    Mammalian

    Mutation

    aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.1-NbON10-NanoLuc

    Plasmid

    #198691

    Purpose

    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, IgK leader sequence, NanoLuc, and twin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

    Plasmid

    #157700

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge