We narrowed to 6,443 results for: plasmid dna

1. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-SARS2-Spike-D614G

   Plasmid

   #166850

   Purpose

   For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2 virus)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA 3.1(-) mouse C/EBP beta (LIP)

   Plasmid

   #12561

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pGRHL2.1.0-gDNA

   Plasmid

   #175863

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   GRHL2 (GRHL2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSRNP3.1.0-gDNA

   Plasmid

   #132472

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CSRNP3 (CSRNP3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHOXB6.1.0-gDNA

   Plasmid

   #132458

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLF10.1.0-gDNA

   Plasmid

   #132433

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   KLF10 (KLF10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRARB.1.0-gDNA

   Plasmid

   #132471

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RARB (RARB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_QLuc

   Plasmid

   #183260

   Purpose

   Express QLuc in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   QLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GtCCR2_EYFP_pcDNA3.1

    Plasmid

    #159121

    Purpose

    This plasmid encodes GtCCR2 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

    Insert

    GtCCR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GtCCR1_EYFP_pcDNA3.1

    Plasmid

    #159120

    Purpose

    This plasmid encodes GtCCR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

    Insert

    GtCCR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MvChR1_EYFP_pcDNA3.1

    Plasmid

    #159119

    Purpose

    This plasmid encodes MvChR1 rhodopsin domain

    Depositor

    John Spudich

    Article

    Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

    Insert

    MvChR1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_SARS2_XBB

    Plasmid

    #194494

    Purpose

    expressing SARS-CoV-2 XBB spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

    Insert

    Spike (XBB) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

    Promoter

    CMV

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4TO-3XFLAG Halo WT nsp1

    Plasmid

    #175412

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1 (ORF1ab CoV2)
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid

    #157716

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3.1-SARS-CoV-2-S-ectodomain

    Plasmid

    #235984

    Purpose

    Internally tagged SARS-CoV-2 spike ecto-domain

    Depositor

    Syed Saif Hasan

    Article

    Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

    Insert

    Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    8xHis and Twin-Strep tag and internal Twin-Strep …

    Expression

    Mammalian

    Mutation

    aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1-NbON10-NanoLuc

    Plasmid

    #198691

    Purpose

    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, IgK leader sequence, NanoLuc, and twin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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