We narrowed to 22,864 results for: Sis

1. Lenti-L227R-ngRNA+15_EF1a-puroR

   Plasmid

   #207360

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR +15 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-V1-rCD20-BSD

   Plasmid

   #209752

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-V2-rCD20-BSD

   Plasmid

   #209753

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDH-V3-rCD20-BSD

   Plasmid

   #209754

   Purpose

   To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

   Promoter

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX307_CR-PDHA2S291D

   Plasmid

   #115200

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307_CR-PDHA2S293D

   Plasmid

   #115201

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307_CR-PDHA2S291D/S293D

   Plasmid

   #115202

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast HA-GPAT4-ΔNs5

   Plasmid

   #173169

   Purpose

   Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Synonymous mutations at sgRNA sites, amino acids …

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

   Plasmid

   #173167

   Purpose

   Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
   

   Use

   Retroviral

   Tags

   3XFLAG

   Expression

   Mutation

   Synonymous mutations at sgRNA sites, G2A, S6A

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-RPS14(WT)-Myc

    Plasmid

    #122236

    Purpose

    expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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