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pFPL-Yg

Plasmid

#61655

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Publication

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Publication

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Publication

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ

Plasmid

#27424

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2-delta-N

Plasmid

#27476

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Publication

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Expression

Bacterial

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP

Plasmid

#42028

Depositor

Kumiko Ui-Tei

Publication

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244079

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244093

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244098

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-S2 (JDW 1529)

Plasmid

#242605

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-S2. H2B for nuclear labeling

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-S2

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-H (JDW 1530)

Plasmid

#242604

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-H. H2B for nuclear labeling

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-H

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244082

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_2-3_005_Promoter+5'UTR_Placeholder

Plasmid

#235870

Purpose

Encodes a sfGFP cassette in the promoter+5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter + 5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_6_026_3C6SF

Plasmid

#235954

Purpose

3' Connector 6 Short Forward in the M2 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

3C6SF

Use

Synthetic Biology

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_005_psbH_3'Hom_Cr

Plasmid

#235939

Purpose

3' Homology psbH in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbH 3'Homology

Use

Synthetic Biology

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-hs-miR-126 (JDW 1401)

Plasmid

#242564

Purpose

Gateway middle entry clone containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artificial intron encoding miR-126 (MIR126 Human)

Use

Gateway Cloning

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-mm-miR-500 (JDW 8)

Plasmid

#242563

Purpose

Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)

Use

Gateway Cloning

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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