We narrowed to 8,835 results for: sgRNA

1. pMXS-IRES-BLAST flag-CAD S1396A

   Plasmid

   #188126

   Purpose

   Expresses C-terminal flag-tagged human CAD S1396A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_NWS.AU1.VSVg_NGFR

   Plasmid

   #158239

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.AU1.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-SwitchON-sgCh2-2

   Plasmid

   #199637

   Purpose

   Tamoxifen-inducible expression of sgRNA control targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Available Since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgCTRL-hygro

   Plasmid

   #199639

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiCRISPR v2-sgACSL4 clone3

   Plasmid

   #162130

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

   Plasmid

   #199263

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO_HA.S.VSVg_mCherry-NLS

   Plasmid

   #178269

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.S.VSVg and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO_HA.HSV.VSVg_mCherry-NLS

   Plasmid

   #178261

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.HSV.VSVg and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid

    #178260

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid

    #178255

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid

    #178246

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO_C.HSV.S_mCherry-NLS

    Plasmid

    #178245

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid

    #178241

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO_C.HA.S_mCherry-NLS

    Plasmid

    #178240

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_C.HA.HSV_mCherry-NLS

    Plasmid

    #178237

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.HSV and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_AU1.HSV.VSVg_mCherry-NLS

    Plasmid

    #178225

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_S.C.V5_NGFR

    Plasmid

    #158331

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.V5

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_AU1.HSV.Ollas_mCherry-NLS

    Plasmid

    #178223

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.Ollas and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_AU1.HA.V5_mCherry-NLS

    Plasmid

    #178221

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HA.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge