We narrowed to 17,085 results for: Por

1. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1043

   Plasmid

   #169621

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS1042

   Plasmid

   #169620

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGB-As-NF-kB1

   Plasmid

   #180209

   Purpose

   Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB1
   

   Use

   Tags

   GAL4 (1-147) fusion

   Expression

   Yeast

   Mutation

   aa 2-409

   Promoter

   AdhI

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-FLAG-As-NF-kB1

   Plasmid

   #180200

   Purpose

   Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB1
   

   Use

   Tags

   FLAG-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 2-409

   Promoter

   CMV

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKT2/CAGXSP/PalmGamillus

   Plasmid

   #182971

   Purpose

   Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   PalmGamillus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-MYC-As-NF-kB2

   Plasmid

   #180204

   Purpose

   Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB2
   

   Use

   Tags

   MYC-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 2-502

   Promoter

   CMV

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-MYC-As-NF-kB1

   Plasmid

   #180203

   Purpose

   Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB1
   

   Use

   Tags

   MYC-tagged fusion protein

   Expression

   Mammalian

   Mutation

   aa 2-409

   Promoter

   CMV

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGB-As-NF-kB2

   Plasmid

   #180210

   Purpose

   Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

   Depositor

   Thomas Gilmore

   Article

   Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

   Insert

   As-NF-kB2
   

   Use

   Tags

   GAL4 (aa 1-147) fusion protein

   Expression

   Yeast

   Mutation

   aa 2-502

   Promoter

   AdhI

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGB-As-NF-kB3

    Plasmid

    #180211

    Purpose

    Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB3
    

    Use

    Tags

    GAL4 (aa 1-147) fusion protein

    Expression

    Yeast

    Mutation

    aa 2-412

    Promoter

    AdhI

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-FLAG-As-NF-kB3

    Plasmid

    #180202

    Purpose

    Expresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    As-NF-kB3
    

    Use

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-412

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG660 lyn11-pFAST

    Plasmid

    #172863

    Purpose

    expresses lyn11-pFAST in mammalian cells (inner membrane labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    Lyn11-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-Hygro-EF1A-RG-Linker-eGFP

    Plasmid

    #176071

    Purpose

    EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    RG
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NmMetQ C20A

    Plasmid

    #172112

    Purpose

    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine on MetQ and Cystein…

    Promoter

    T7 Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPC002

    Plasmid

    #171139

    Purpose

    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    J1-BBa_J23117 and Sp.pCas9, BBa_J23107

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-uTEV1-MAVS-mTA2

    Plasmid

    #171000

    Purpose

    HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[R537A, R538A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-uTEV1-MAVS-mTA3

    Plasmid

    #171001

    Purpose

    HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIV]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-uTEV1-MAVS-mTA5

    Plasmid

    #171003

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI, R537A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

    Plasmid

    #166410

    Purpose

    express BirA-hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    POT1 ΔOB (POT1 Human)
    

    Use

    Tags

    MYC-flag-BirA

    Expression

    Mammalian

    Mutation

    ΔOB

    Promoter

    cmv

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

    Plasmid

    #164132

    Purpose

    Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    ciCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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