We narrowed to 12,637 results for: NSI
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Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgCIC-1
Plasmid#74953PurposeCas9 + sgCIC-1 with blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-mCherry
Plasmid#140306PurposeExpresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FLExpressionMammalianMutationcontains only the SH4 and UD regions fused to mCh…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
Plasmid#176250PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozymDepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-oROS-HT
Plasmid#216416PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.DepositorInsertpDisplay-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FYN-1/1
Plasmid#91413PurposeProtein expression and purification of human SH3 domain construct FYN-1/1DepositorInsertFYN-1/1 (FYN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-ArchT-GFP
Plasmid#58851PurposeAAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertArchT-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianPromoterEF1aAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)
Plasmid#68218PurposeModule composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expressionDepositorInsertLuc / Renilla / P19
UseLuciferase and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos / 35S / 35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
TagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2
Plasmid#169223PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nsp14-mCherry
Plasmid#165137Purposemammalian expression and localizationDepositorAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_NR1_NR2_LPT1
Plasmid#176253PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT geneDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-UD-S32
Plasmid#140303PurposeExpresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationlacks the KD regionPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pg-proPHD2-PHD2-EGFP
Plasmid#87210PurposePHD2-EGFP expressed under the control of the PHD2 promoterDepositorAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP-CAAX
Plasmid#208684PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neuronsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseAAVPromoterhSynAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Flag-LplA
Plasmid#232118PurposeFor bacterial expression of Flag-tagged E. coli LplA.DepositorInsertlplA (lplA Escherichia coli (strain K12))
TagsFlagExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD45
Plasmid#203753PurposeEncodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLXSN 5myc-CDC6-mut
Plasmid#109333Purposeexpress tagged Cdc6 mutantDepositorInsertCDC6 (CDC6 Human)
UseRetroviralTags5x MycExpressionMammalianMutationR56A, L59A, K81A, E82A, N83AAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-3xA
Plasmid#101041PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
TagsGFPExpressionMammalianMutationS1915A, S1916A, S1943APromoterCMVAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-NpHR3.3-EYFP
Plasmid#137151PurposeViral expression of NpHR3.3-EYFPDepositorHas ServiceAAV8InsertNpHR3.3-EYFP
UseAAVMutationW179FPromoternEFAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only