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pYPQ-dpcoCas9-TV

Plasmid

#158409

Purpose

CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-TV(6xTAL-2xVP64)

Use

CRISPR

Expression

Plant

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#158600

Purpose

Transcription factors ADR1c, PIP2c, OAF1c

Depositor

John Dueber

Publication

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

ADR1c
PIP2c
OAF1c

Expression

Yeast

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-mCherry

Plasmid

#114275

Purpose

Ubiquitous expression of the mCherry living color

Depositor

Patrick Salmon

Publication

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

mCherry

Use

Lentiviral

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131C2.0

Plasmid

#99886

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pYPQ132C2.0

Plasmid

#99889

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMZ377

Plasmid

#74214

Purpose

Combined sgRNA/Cas9 vector with LEU2 marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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LIIalpha F 1-2 RNAi

Plasmid

#54404

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI dy F-G

Plasmid

#54345

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

F-G dummy

Use

Cloning vector

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pHnCB

Plasmid

#52064

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducible

Depositor

David Savage

Publication

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B

Expression

Bacterial

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTCS-mcs

Plasmid

#31288

Depositor

Dirk Schnappinger

Publication

Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Publication

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR_EF1a-->myc-mtC11L34

Plasmid

#162226

Purpose

Lentiviral vector for the expression of Myc mtC11L34 in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-PNE scFv C11L34

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Publication

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Expression

Bacterial

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-LO

Plasmid

#162754

Purpose

Retroviral (MSCV) expression of LSSmOrange fluorescent protein

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

LSSmOrange

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-AM

Plasmid

#162751

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PrpoN-CroAct3-PBBa_J23106-geneIII

Plasmid

#134354

Purpose

Phagemid

Depositor

Mark Isalan

Publication

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

CroAct3

Expression

Bacterial

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Publication

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAct:MS2-p65-HSF

Plasmid

#78902

Purpose

Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-p65-HSF

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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