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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 79,221 results for: rest

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Showing: 8081 - 8100 of 79221 results

  3. pcDNA 3.1 SIRT1-FL

    Plasmid
    #105670
    Purpose
    Mammalian expression construct of mouse SIRT1 full length isoform
    Depositor
    Ullas Kolthur-Seetharam
    Publication
    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SIRT1 (Sirt1 Mouse)
    Expression
    Mammalian
    Mutation
    Synonymous base changes not affecting the protein…
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105670-plasmid-map-sequence-id-207902

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  4. pcDNA3-pmEKAR4

    Plasmid
    #174440
    Purpose
    Plasma membrane-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174440-plasmid-map-sequence-id-344974

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  5. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Publication
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108692-plasmid-map-sequence-id-208976

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  9. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74492-plasmid-map-sequence-id-140493

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  20. pcDNA3.1_crGE

    Plasmid
    #172933
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.
    Depositor
    Arnold Boersma
    Publication
    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CrowdingSensorGE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172933-plasmid-map-sequence-id-339481

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Showing: 8081 - 8100 of 79221 results