We narrowed to 63,254 results for: cloning

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - P381L in pENTR

Plasmid

#216570

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

P381L

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - D424N in pENTR

Plasmid

#216571

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

D424N

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM042_P15678_MultiGeneBackbone

Plasmid

#216467

Purpose

Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM041_P15678_SingleGeneBackbone

Plasmid

#216466

Purpose

Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM032_P4_tSynth30

Plasmid

#216457

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth30

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM031_P4_tSynth29

Plasmid

#216456

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth29

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM030_P4_tSynth27

Plasmid

#216455

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth27

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM029_P4_tSynth25

Plasmid

#216454

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth25

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM028_P4_tSynth3

Plasmid

#216453

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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