We narrowed to 13,805 results for: gan

1. CAHS2 (pBS0249)

   Plasmid

   #185139

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2
   

   Expression

   Mammalian

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6M-PAK1 H83L H86L K299R

   Plasmid

   #26592

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   H83L H86L K299R Dominant negative

   Available Since

   March 9, 2011

   Availability

   Academic Institutions and Nonprofits only

3. CMV-FlicR1

   Plasmid

   #74142

   Purpose

   Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCYOX1L-bio-His

   Plasmid

   #53410

   Purpose

   Expresses full-length Prenylcysteine oxidase-like precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PCYOX1L (PCYOX1L Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Neurl1-Flag pcDNA3.1

   Plasmid

   #17282

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Neuralized-like (Neurl1a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pB-MultiplexedReporter-MOR170-1

   Plasmid

   #129461

   Purpose

   piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   MOR170-1 (Olfr895 Mouse)
   

   Use

   Synthetic Biology

   Tags

   LUCY tag, Rho tag

   Expression

   Mammalian

   Promoter

   TRE (Tet-On)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-SARS-CoV2-NP

   Plasmid

   #165090

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Lenti-FAB(mito-LD)

   Plasmid

   #214421

   Purpose

   Detection of mito-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-3XHA-V5

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIRECT_25H

   Plasmid

   #91145

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-NPC1(P202A/F203A)-FLAG

    Plasmid

    #164976

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P202A/ F203A

    Promoter

    EF1a

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SP-GFP-CD274-NU

    Plasmid

    #121139

    Purpose

    Mammalian expression of GFP tagged CD274

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Inserts

    CD274 (CD274 Human)
    CD274 (CD274 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDIRECT_21C

    Plasmid

    #91130

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV BARK D110A p2A mCherry

    Plasmid

    #117690

    Purpose

    CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    D110A

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1 3xFlag-MAPKAPK2

    Plasmid

    #82720

    Purpose

    Expresses human MAPKAPK2

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MAPKAPK2 (MAPKAPK2 Human)
    

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

18. rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

    Plasmid

    #32477

    Purpose

    Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAML141F,Y115F-5HT3HC
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Promoter

    Synapsin

    Available Since

    Jan. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG94-1--LaG94-14

    Plasmid

    #228973

    Purpose

    For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-1--LaG94-14
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaTdT-1

    Plasmid

    #228974

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-1
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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