We narrowed to 18,501 results for: MUT

1. pRK5 LYCHOS (GPR155) Y551A-HA

   Plasmid

   #199676

   Purpose

   Transient expression of LYCHOS (GPR155) Y551A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y551 mutated to A

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-Nedd1-mOrange2-F75A

   Plasmid

   #196862

   Purpose

   Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-F75A (Nedd1 Rat)
   

   Expression

   Mammalian

   Mutation

   F75A in the CM1 domain

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-LYCHOS (GPR155) P44A-HA

   Plasmid

   #199675

   Purpose

   Transient expression of LYCHOS (GPR155) P44A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P44 mutated to A

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-LYCHOS (GPR155) E48Q-HA

   Plasmid

   #199662

   Purpose

   Transient expression of LYCHOS (GPR155) E48Q mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E48 mutated to Q

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-LYCHOS (GPR155) F43I-HA

   Plasmid

   #199674

   Purpose

   Transient expression of LYCHOS (GPR155) F43I mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F43 mutated to I

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-861_R

   Plasmid

   #147404

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-861

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-861 (gw Nematode, Fly)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_635-1384_R

   Plasmid

   #147405

   Purpose

   Insect Expression of CeAIN2_254-400-DmGW182_635-1384

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

   Insert

   CeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)
   

   Expression

   Insect

   Mutation

   Deletion of 2 AA (SD) at position 378-379 compare…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN-A112C-CP8B1

   Plasmid

   #191240

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
   

   Tags

   nuclease A from S. aureus fused to the poly-Leu T…

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and predic…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pITGA5_1745G>U

   Plasmid

   #173812

   Purpose

   mir218 binding site2 point mutation

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   NT1745 ITGA5 point mutation (ITGA5 Human)
   

   Expression

   Mammalian

   Promoter

   AmpR

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pITGA5_473G>A

    Plasmid

    #173811

    Purpose

    mir218 binding site1 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT473 ITGA5 point mutation (ITGA5 Human)
    

    Expression

    Mammalian

    Promoter

    AmpR

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pITGA5_Q853L

    Plasmid

    #174008

    Purpose

    Fibronectin receptor binding site point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    FibronR ITGA5 point mutation (ITGA5 Human)
    

    Expression

    Mammalian

    Promoter

    AmpR

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LeGO-iBSD-5LO-E376Q

    Plasmid

    #182742

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase E376Q (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    enzymatically inactive 5-LO E376Q mutant, codon o…

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBoBi-hPEN2 D90A

    Plasmid

    #183641

    Purpose

    Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 90 of PEN2 mutated to alanine

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBoBi-hPEN2 2A

    Plasmid

    #183640

    Purpose

    Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 35 and 40 of PEN2 mutated to alanine

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Phage-PGK-Flag-HA-FKBP-T(G177D)

    Plasmid

    #181735

    Purpose

    for lentiviral expression of brachyury ORF in mammalian cells

    Depositor

    Charles Lin

    Article

    Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

    Insert

    T (brachyury)
    

    Use

    Lentiviral

    Mutation

    a silent mutation in the PAM site of an sgRNAt ta…

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid

    #129793

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    G315T

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid

    #129789

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    W99A

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid

    #129790

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    N51L

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EIF2B3-C424G

    Plasmid

    #172091

    Purpose

    In vitro transcription for rescue experiments

    Depositor

    Cheol-Hee Kim

    Article

    Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

    Insert

    EIF2B3 (EIF2B3 Human)
    

    Expression

    Mammalian

    Mutation

    c.1270T>G

    Promoter

    CMV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EIF2B3-V398fs

    Plasmid

    #172090

    Purpose

    In vitro transcription for rescue experiments

    Depositor

    Cheol-Hee Kim

    Article

    Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

    Insert

    EIF2B3 (EIF2B3 Human)
    

    Expression

    Mammalian

    Mutation

    c.1193-1194delTG

    Promoter

    CMV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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