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pXPR_124

Plasmid

#133461

Purpose

EF1a promoter expresses SpyoCas9 and P2A provides EGFP

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

SpyoCas9

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1574

Plasmid

#119178

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS1

Expression

Bacterial

Promoter

pGAP

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pME-6MTnlstdTomato2A

Plasmid

#67685

Purpose

Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

tdTomato

Tags

2A and 6xMyc-NLS

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pT-BS

Plasmid

#31561

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

LoxP

Expression

Bacterial

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pJS1

Plasmid

#253726

Purpose

CRISPRi system for Pseudomonas aeruginosa expressing dcas9 from the rhamnose-inducible rhaBAD promoter

Depositor

Deborah Hung

Article

Sullivan et al mBio. 2026 Feb 11:e0276725. doi: 10.1128

Insert

dcas9

Tags

6x his

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pPOP-BsmBI

Plasmid

#241281

Purpose

Cloning gene libraries from oligonucleotide pools

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Type

Empty backbone

Use

Other

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPOP-BbsI

Plasmid

#241282

Purpose

Cloning gene libraries from oligonucleotide pools

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Type

Empty backbone

Use

Other

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxKOk

Plasmid

#229949

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pR_Lux

Plasmid

#226457

Purpose

lambda immunity region to the luciferase operon (lux)

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens

Expression

Bacterial

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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MPRA Empty Vector

Plasmid

#204865

Purpose

Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pReg-CamR

Plasmid

#190615

Purpose

Expresses the camphor-responsive biosensor CamR in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al ACS Synth Biol. 2022 Jan 5. doi: 10.1021

Insert

CamR

Use

Synthetic Biology

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFKBPmCherry-Akt2(KD)

Plasmid

#86598

Purpose

Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

FKBP-mCherry-Akt2(KD)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUC12

Plasmid

#50002

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Type

Empty backbone

Expression

Bacterial

Promoter

lac

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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mEos-A69V-C1

Plasmid

#54647

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEos A69V

Expression

Mammalian

Available Since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMartini Gate A R2-R1

Plasmid

#36428

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Insert

Gateway Cassette A R2-R1

Use

Gateway conversion vector

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pMartini Gate C R1-R2

Plasmid

#36435

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Insert

Gateway Cassette

Use

Gateway conversion vector

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CMV-eBRIC

Plasmid

#241800

Purpose

Bioluminescence indicator for Ca2+ imaging in mammalian cells

Depositor

Huiwang Ai

Article

Tian et al ACS Sens. 2025 Jul 29. doi: 10.1021/acss

Insert

eBRIC

Expression

Bacterial

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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