We narrowed to 9,365 results for: tre promoter

1. pEF5-H4Δ1-19-HaloTag-FRT

   Plasmid

   #247456

   Purpose

   Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

   Depositor

   Kazuhiro Maeshima

   Article

   Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

   Insert

   H4 (H4C1) (H4C1 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   H4Δ1-19 lacks the initial 19 residues at the N-te…

   Promoter

   EF1α

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCBE3-HF1-Hypa

   Plasmid

   #218158

   Purpose

   This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTetON-DHFRdd-SpvB; CMV-mCherry

   Plasmid

   #89463

   Purpose

   Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   E. coli DHFRdd destabilization domain and mCherry…

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSc1

   Plasmid

   #80436

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT MetNIQ in pBAD

   Plasmid

   #118253

   Purpose

   Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 295 to Alanine (Ala)

   Promoter

   None and araBAD Promoter

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSc2

   Plasmid

   #80437

   Purpose

   Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

   Depositor

   Ervin Welker

   Article

   Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

   Inserts

   sp Cas9 gRNA
   eGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5-H4(A5,8,12,16)-HaloTag-FRT

   Plasmid

   #247455

   Purpose

   Expresses wild-type H4(A5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

   Depositor

   Kazuhiro Maeshima

   Article

   Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

   Insert

   H4 (H4C1) (H4C1 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   H4(A5,8,12,16) has four mutations in the N-termin…

   Promoter

   EF1α

   Available Since

   Nov. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5-H4(Q5,8,12,16)-HaloTag-FRT

   Plasmid

   #247454

   Purpose

   Expresses wild-type H4(Q5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

   Depositor

   Kazuhiro Maeshima

   Article

   Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

   Insert

   H4 (H4C1) (H4C1 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   H4(Q5,8,12,16) has four mutations in the N-termin…

   Promoter

   EF1α

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF5-H4(R5,8,12,16)-HaloTag-FRT

   Plasmid

   #247453

   Purpose

   Expresses wild-type H4(R5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

   Depositor

   Kazuhiro Maeshima

   Article

   Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

   Insert

   H4 (H4C1) (H4C1 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   H4(R5,8,12,16) has four mutations in the N-termin…

   Promoter

   EF1α

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCBE3-NG-HF1

    Plasmid

    #218160

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCBE3-NG-Hypa

    Plasmid

    #218161

    Purpose

    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. peSCBE3-NG-Hypa

    Plasmid

    #218165

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. peSCBE3-NG-HF1

    Plasmid

    #218164

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. N229A (EcQ) MetNIQ in pBAD

    Plasmid

    #118257

    Purpose

    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Asparagine (Asn) 229 to Alanine (Ala) and…

    Promoter

    None and araBAD Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid

    #118260

    Purpose

    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    

    Tags

    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

    Promoter

    None and araBAD Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCBE3-NG-HF1-Hypa

    Plasmid

    #218162

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet-TFIIE

    Plasmid

    #171083

    Purpose

    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Inserts

    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NanoBRET BiBRET Vector HaloTag-KRAS 2B WT-BRAF RBD-NanoLuc

    Plasmid

    #236853

    Purpose

    Express HaloTag(R)-KRAS 2B WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCB268

    Plasmid

    #53363

    Purpose

    pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

    Depositor

    Alexander Varshavsky

    Article

    Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

    Inserts

    DHFR (Dhfr Mouse)
    Ub
    Aβ42 (APP Human)
    

    Tags

    13xMyc, Flag, and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. NanoBRET BiBRET Vector, NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag

    Plasmid

    #238576

    Purpose

    Express NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    CRAF(RBD-CRD) (RAF1 Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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