We narrowed to 3,113 results for: GAI

1. FLAG-Foxo1ADA(pCMV5)

   Plasmid

   #12149

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   Threonine 24 to Alanine (A) and Serine 253 to Asp…

   Promoter

   CMV

   Available since

   June 13, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-CaMKII-jGCaMP7f-WPRE

   Plasmid

   #203848

   Purpose

   Expression of GCaMP7f in CaMKII expressing cells

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   jGCaMP7f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV asyn WT

   Plasmid

   #36055

   Depositor

   Hilal Lashuel

   Article

   Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

   Insert

   alpha-synuclein WT (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIX_sh_HOIP/RNF31_#6

   Plasmid

   #75252

   Purpose

   retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against human RNF31 (RNF31 )
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-Foxo1-WT 6KQ

   Plasmid

   #17562

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 6KQ (Foxo1 Mouse)
   

   Use

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   K242, K245, K259, K262, K271 and K291 were replac…

   Promoter

   CMV

   Available since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pTorPE-R-GECO1

   Plasmid

   #32465

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Use

   Lab constructed

   Tags

   6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

   Expression

   Bacterial

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-mito-CAR-GECO1

   Plasmid

   #46022

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2-7xTcf-NLS-CNL

    Plasmid

    #65712

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-GEX-GECO1

    Plasmid

    #32443

    Purpose

    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-FLAG-MYO1D

    Plasmid

    #125125

    Purpose

    Expresses human FLAG-MYO1D

    Depositor

    Bruce Beutler

    Article

    McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

    Insert

    MYO1D (MYO1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVUTHshGATA1-tTR-KRAB

    Plasmid

    #11650

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT/FLAG/HA-DEST EIF2C2

    Plasmid

    #19888

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

    Plasmid

    #110556

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    anit-RD114 light chain
    

    Use

    Tags

    mKappa

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2xFLAGhYAP2

    Plasmid

    #17793

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

    Plasmid

    #181742

    Purpose

    AAV vector for expressing CFL-SN and GCaMP6f

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    CFL-SN-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-Flex-mRuby3-2A

    Plasmid

    #203846

    Purpose

    Neuron specific expression of a Cre-dependent mRuby3

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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