We narrowed to 1,452 results for: NEB

Showing: 801 - 820 of 1452 results

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pScarlessHD-N-3x127D01-DsRed

Plasmid

#171579

Purpose

For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV neo KD Akt1

Plasmid

#14550

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt1 KD (Akt1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Kinase Dead Mutant: K179M

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-n

Plasmid

#53077

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.0-T2A-EGFP

Plasmid

#216488

Purpose

Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.0

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-PH-TAPP1

Plasmid

#161985

Purpose

Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

Depositor

Dario Alessi, David Bryant

Article

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-HA-His

Plasmid

#171567

Purpose

Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-HA-His (UBE2J1 Human)

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-Nb127D01-GFP

Plasmid

#171571

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-GFP (CXCR2 Human)

Tags

GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-Nb127D01-mCherry

Plasmid

#171573

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-mCherry (CXCR2 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-NbVHH05-GFP

Plasmid

#171576

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-NbVHH05-GFP (UBE2J1 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDTFendoNA2

Plasmid

#174731

Purpose

Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.

Depositor

Jukka Finne

Article

Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

Insert

DT386F

Expression

Bacterial

Mutation

Deleted amino acids 412-560 (the receptor binding…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-c

Plasmid

#53072

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-end-c

Plasmid

#53074

Purpose

destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBullet-cg-c

Plasmid

#53070

Purpose

destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-vac-c

Plasmid

#53076

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-er-c

Plasmid

#53068

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-cyt-c

Plasmid

#53066

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-cg-n

Plasmid

#53069

Purpose

destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-end-n

Plasmid

#53073

Purpose

destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge