We narrowed to 313,530 results for: addgene

1. 6V41

   Plasmid

   #162272

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   CDY1 (CDY1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 6WAU

   Plasmid

   #162269

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   PHF19 (PHF19 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. 6WAJ

   Plasmid

   #162267

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   NLE1 (NLE1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. 6OGN

   Plasmid

   #162260

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   PRMT7 (PRMT7 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCAMTA2.1.0-gDNA

   Plasmid

   #132456

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   CAMTA2 (CAMTA2 Human)
   

   Use

   CRISPR

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBD1.1.0-gDNA

   Plasmid

   #132444

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   MBD1 (MBD1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6NIM

   Plasmid

   #127294

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD18
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 26, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 5VS7 (PP-BRD4)

   Plasmid

   #101264

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD4
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. 5KO4 (PP-BRD19)

   Plasmid

   #101882

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD19
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 5TCK (PP-BRD3)

    Plasmid

    #101887

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 5U9N (PP-BRD3)

    Plasmid

    #101255

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 4QNY

    Plasmid

    #61958

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LdBPK_331470.1:MAC04E-A10:C228347
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. PP-MAPK2 (3N9X)

    Plasmid

    #28117

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PBG-PF11_0147
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

14. PP-CDPK3

    Plasmid

    #28151

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDPK3
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    CAD domain (residues 425-602).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-PB-R4R3

    Plasmid

    #139518

    Purpose

    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector

    Depositor

    Minoru Ko

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. RedTrackCMV

    Plasmid

    #50957

    Purpose

    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP

    Depositor

    James Bamburg

    Type

    Empty backbone

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

    Plasmid

    #110560

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-lacZ
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Promoter

    TRE

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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