Addgene

We narrowed to 5,876 results for: pcDNA 3.1

Showing: 801 - 820 of 5876 results

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pcDNA3.1_C-6His-Stx17

Plasmid

#112259

Purpose

C-term His tagged pcDNA3.1 vector expressing mouse Stx17 (syntaxin 17)

Depositor

Madhulika Tripathi

Insert

syntaxin 17 (Stx17 Mouse)

Use

Tags

6His Tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

Plasmid

#148118

Purpose

Mammalian Expression of HshnRNPE2iso3

Depositor

Elisa Izaurralde

Insert

HshnRNPE2iso3 (PCBP2 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation L239 to S compapred to th…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-4LNDDE488Q-pCDNA3.1(+)

Plasmid

#172314

Purpose

Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

Depositor

Joshua Rosenthal

Article

Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

Insert

Lambda N peptide - human ADAR2 Deaminase Domain E488Q

Use

Tags

6xHis, Flag, and Nuclear Localization Signal of S…

Expression

Mammalian

Mutation

changed the Glutamic acid 488 in the Deaminase Do…

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKI-GFP

Plasmid

#118482

Purpose

GFP-tagged Protein Kinase A inhibitor peptide.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

PKI-GFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-YFP-KRAS4B

Plasmid

#112718

Purpose

Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

YFP-KRAS4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
CrBlue-mcherry - pcDNA3.1

Plasmid

#64204

Purpose

CrBlue fused to mcherry on the C terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115

Plasmid

#181996

Purpose

Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115 (ARHGEF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM4-Halo-FRB

Plasmid

#197057

Purpose

Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM7-Halo-FRB

Plasmid

#197058

Purpose

Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM22-Halo-FRB

Plasmid

#197059

Purpose

Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FRB-GEM23-Halo

Plasmid

#197060

Purpose

Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

I3-01

Use

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_FLAG-pcDNA 3.1

Plasmid

#73593

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-Sesn2

Plasmid

#61868

Purpose

Transient overexpression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-3xFLAG-RSL1D1(WT)

Plasmid

#122301

Purpose

expresses RSL1D1 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-V5/HIS

Plasmid

#201985

Purpose

expression of human FLT3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

FLT3 receptor tyrosine kinase (FLT3 Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CnA-H151A

Plasmid

#191956

Purpose

Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).

Depositor

Luca Scorrano

Insert

Calcineurin subunit alpha H151A

Use

Tags

Expression

Mammalian

Mutation

H151A

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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