We narrowed to 1,235 results for: sumo

1. pRSF-KpAGO207-1251

   Plasmid

   #117854

   Purpose

   Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

   Depositor

   Kotaro Nakanishi, Dinshaw Patel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Argonaute
   

   Use

   Tags

   6xHis-SUMO encoded in vector

   Expression

   Bacterial

   Mutation

   missing first 206 amino acid residues

   Promoter

   T7

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCri-11b

   Plasmid

   #61333

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 5UBB (LOC149281)

   Plasmid

   #101257

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   METTL11B (METTL11B Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

   Plasmid

   #228633

   Purpose

   Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Enterovirus Coxsackievirus A71 2A protease
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   C110A

   Promoter

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pEPQD0CM0549

   Plasmid

   #162300

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 6DUB (LOC149281)

   Plasmid

   #119967

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   LOC149281 (METTL11B Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 58-278

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCri-11a

   Plasmid

   #61319

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

8. hUbc13

   Plasmid

   #51131

   Purpose

   human ubc13, E2 for synthesis k63 linked ubiquitin chain

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

   Insert

   ubc13 (UBE2N Human)
   

   Use

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pESC-URA-GFP-Ubc9ts

   Plasmid

   #20369

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y68L This mutation renders Ubc9 temperature-sens…

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pQA-LP574

    Plasmid

    #222953

    Purpose

    Expresses R120A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Use

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 120 to Alanine

    Promoter

    T7

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX3X-RanBP2dFG

    Plasmid

    #53120

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2553-2838)

    Promoter

    tac

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-Sp100A(K297A)-C1

    Plasmid

    #134552

    Purpose

    Expresses YFP-Sp100A fusion protein with a K297A mutation

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2013 Mar 13.

    Insert

    Sp100A(K297A)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K297R point mutation in the SUMO site

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIA1400

    Plasmid

    #172519

    Purpose

    Expression of SARS-COV-2 Nsp12 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIA1402

    Plasmid

    #172403

    Purpose

    Expression of SARS-COV-2 Nsp12 Y129A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Y129A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIA1408

    Plasmid

    #172405

    Purpose

    Expression of SARS-COV-2 Nsp12 D760A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D760A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT-36

    Plasmid

    #16252

    Depositor

    Yoshiko Kikuchi

    Article

    Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

    Insert

    CDC3 (CDC3 Budding Yeast)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only