We narrowed to 9,611 results for: bli

1. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

   Plasmid

   #223721

   Purpose

   Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted sterile alpha motif domain (deleted amino…

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

   Plasmid

   #214468

   Purpose

   Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S14D S25D S32D T261D

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

   Plasmid

   #214471

   Purpose

   Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   ZFYVE27 (ZFYVE27 Human)
   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S14D S25D S32D T261D

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-Creb5(HA)

   Plasmid

   #195022

   Purpose

   Gateway vector containing HA-tagged Creb5

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
   

   Use

   Gateway cloning

   Tags

   3xHA

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GL4.10 E2E1- Prg4 promoter-luciferase

   Plasmid

   #195035

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
   

   Use

   Luciferase

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.10 E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195036

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
   

   Use

   Luciferase

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195037

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
   

   Use

   Luciferase

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus-Bik-del72-136-pEGFP-C3

   Plasmid

   #191411

   Purpose

   Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).

   Depositor

   David Andrews

   Insert

   Bcl-2-interacting killer (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   with deletion of amino acids 72-136 (del72-136) i…

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

   Plasmid

   #140454

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, tyrosine 616 to methioni…

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid

    #140455

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, leucine 624 to asparagine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid

    #140456

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, alanine 634 to asparagine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid

    #140449

    Purpose

    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    FLAG tag, Signal/leader sequence from HLA class I…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid

    #140450

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid

    #140452

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, isoluecine 578 to alanine

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB53-Pac-tetO-mCherry-attB53

    Plasmid

    #183612

    Purpose

    RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tetO-mCherry
    

    Use

    Rmce

    Promoter

    tetO

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid

    #158616

    Purpose

    B6J Id1-LSL-DTA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Mutation

    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-MIOX only

    Plasmid

    #166683

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    GFP-MIOX
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTEF1-UDH

    Plasmid

    #166679

    Purpose

    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    UDH
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. wtVP1 only

    Plasmid

    #166673

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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