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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,905 results for: sta

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Showing: 8281 - 8300 of 14905 results

  1. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124807-plasmid-map-sequence-id-251832

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  5. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91733-plasmid-map-sequence-id-180936

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  6. pCA-ATG-intron-tdT3Myc

    Plasmid
    #36883
    Depositor
    Liqun Luo
    Publication
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato-3Myc
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    beta-globin intron containing a loxP site inserte…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer)
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    36883-plasmid-map-sequence-id-46133

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  8. pKIF1C-2xFLAG

    Plasmid
    #130979
    Purpose
    Expression of KIF1C-2xFLAG in mammalian cells.
    Depositor
    Anne Straube
    Publication
    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-2xFLAG (KIF1C Human)
    Tags
    2xFLAG
    Expression
    Mammalian
    Mutation
    4 silent mutations that make this construct resis…
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130979-plasmid-map-sequence-id-259357

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  9. pANAP-P2A-eRF(E55D)

    Plasmid
    #241290
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor
    Depositor
    Justin Taraska
    Publication
    Obashi et al Structure. Volume 34, Issue 1, 8 January
    Insert
    the amber suppressor tRNACUA-Leu
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV
    Available since
    Dec. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241290-plasmid-map-sequence-id-481082

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  10. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  12. phROSA26-Tet-HA-JLP_WT

    Plasmid
    #208770
    Purpose
    Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus
    Depositor
    Katsuji Yoshioka
    Publication
    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JLP (SPAG9 Human)
    Expression
    Mammalian
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    208770-plasmid-map-sequence-id-412876

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  15. pLenti-CD74-ROS1 L2000V

    Plasmid
    #183815
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cells
    Depositor
    Aaron Hata
    Publication
    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    L2000V
    Promoter
    CMV
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183815-plasmid-map-sequence-id-360442

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  16. mILK-R255A/R349A-EFGP

    Plasmid
    #176899
    Purpose
    mouse ILK with R255A and R349A point mutations cloned into pEGFP-N1
    Depositor
    Sara Wickström
    Publication
    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Integrin-linked kinase (Ilk Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Arginine 255 to Alanine and Arginine 349 to Alani…
    Promoter
    CNV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176899-plasmid-map-sequence-id-348233

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  17. pGBW-m4046907

    Plasmid
    #149262
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149262-plasmid-map-sequence-id-358322

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  18. pGBW-m4046735

    Plasmid
    #149234
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 ORF8 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF8 protein (ORF8 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149234-plasmid-map-sequence-id-332773

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  19. pGBW-m4046788

    Plasmid
    #145675
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145675-plasmid-map-sequence-id-307564

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  20. pGBW-m4046796

    Plasmid
    #145666
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145666-plasmid-map-sequence-id-308762

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Showing: 8281 - 8300 of 14905 results