We narrowed to 13,467 results for: sequence
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Plasmid#133331PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]
Plasmid#133332PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMACHis6_PiuD
Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
Tags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]
Plasmid#118813PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationG368W and W385G in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
Plasmid#118808PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll F to S in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]
Plasmid#118809PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]
Plasmid#118810PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds
Plasmid#119070PurposeConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled1-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds
Plasmid#119071PurposeConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds
Plasmid#119072PurposeConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled3-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds
Plasmid#119073PurposeConstruct with scrambled-4 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled4-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP
Plasmid#114730PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M7
UseCRISPRExpressionMammalianAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI041
Plasmid#105414PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-hTtAgo
Plasmid#104383Purposehuman codon optimized TtAgo; pCDNA3 vector backbone, mammalian expressionDepositorInserthTtAgo
Tags3XFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMElmo2P1gfp
Plasmid#90127PurposeGateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFPDepositorAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p5Edusp5E1
Plasmid#90122Purpose5' Gateway entry clone containing endothelial specific enhancer sequence of dusp5DepositorInsertdusp5E1 (dusp5 Zebrafish)
ExpressionBacterialAvailable SinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only