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pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Tags

tdTomato

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-mHRI-FLAG-IP

Plasmid

#101791

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse HRI

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Hri (Eif2ak1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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YTIP-GFP

Plasmid

#42238

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YTIP-GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMIR-9

Plasmid

#25037

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 binding site (MIR9-1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
XE117 CAMKII-CS2+

Plasmid

#16737

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII (Camk2a Rat)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pPRIME-CMV-LNGFR-FF3

Plasmid

#11666

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

Tre-3G

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-PCV

Plasmid

#123644

Purpose

Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

2-116

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-T1ER

Plasmid

#47928

Purpose

T1ER is a calcium sensor targeted to ER

Depositor

Tobias Meyer

Article

Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

Insert

T1ER

Expression

Mammalian

Mutation

KDEL

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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haA1.pBABEpu

Plasmid

#21813

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 C-term

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pPRIME-TREX-GFP-FF3

Plasmid

#11667

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP Raptor

Plasmid

#12587

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pET 15b-UbcH5a/2417

Plasmid

#11472

Depositor

Allan Weissman

Article

Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

Insert

UbcH5a (UBE2D1 Human)

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GGamma13-GFP2

Plasmid

#140992

Purpose

Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma13-GFP2 (GNG13 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ12: CadC-VHH-Caffeine (No linker)

Plasmid

#108244

Purpose

Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker

Use

Synthetic Biology

Promoter

pLacO1

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX-RCC1-HA

Plasmid

#106938

Purpose

Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

Insert

human RCC1-HA (RCC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDS24

Plasmid

#58952

Purpose

Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

Depositor

Thomas Fox

Article

Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

Insert

Mitochondrially coded Arg8 protein

Mutation

Coding sequence synthetic in S. cerevisiae mitoch…

Promoter

n/a

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only