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pLX-EF1a_NKX2-4

Plasmid

#221490

Purpose

NKX2-4 lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-4 (NKX2-4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Publication

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Promoter

CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TRE3GS-eGFP

Plasmid

#252871

Purpose

TRE3GS expression plasmid for Flp/FRT recombination expressing eGFP

Depositor

Norman Davey

Insert

eGFP

Expression

Mammalian

Promoter

TRE3GS

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP-VAV1-W495L

Plasmid

#242839

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Publication

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

W495L

Available since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1-E201A

Plasmid

#242838

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Publication

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

E201A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-WASP-3D

Plasmid

#242836

Purpose

Encodes human WASP and N-terminal EGFP tag

Depositor

Cheng-han Yu

Publication

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

WASP (WAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

F244D, H246D, H249D

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1101

Plasmid

#235708

Purpose

Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

Depositor

Roger Williams

Publication

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
ATG14L (ATG14 Human)

Expression

Mammalian

Promoter

CAG and CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-RNF126 C13,16A

Plasmid

#138645

Purpose

Bacterial expression of GST-RNF126 ZnF mutant (C13,16A) fusion protein

Depositor

Jane McGlade

Publication

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C13A, C16A

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO-1_S168A

Plasmid

#259708

Purpose

For introducing EGFP-ZO-1_S168A into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S168 is substituted with A

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pET-LIC-cloning vector (2A-T) No Golden Gate sites BsaI and Esp3I

Plasmid

#260643

Purpose

LIC cloning vector with Golden Gate sites BsaI and Esp3I removed

Depositor

Stan Fields

Publication

Ashok et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-TagRFP-T-EEA1

Plasmid

#254776

Purpose

Neutrophil specific Early Endosome Antigen 1 expression in tol2 backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

EEA1 (EEA1 Human)

Use

Zebrafish expression

Tags

TagRFP-T

Promoter

Lyzc

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 locus donor plasmid

Plasmid

#252870

Purpose

Plasmid encoding FRT recombination site and TetOn3G transactivator flanked by AAVS1 homology arms

Depositor

Norman Davey

Insert

TetOn3G transactivator and FRT recombination site

Expression

Mammalian

Available since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_CSF1R

Plasmid

#187767

Purpose

MAC-tagged gene expression of human CSF1R

Depositor

Markku Varjosalo

Publication

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

CSF1R (CSF1R Human)

Expression

Mammalian

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-yVps23-UEV_C133A

Plasmid

#45924

Depositor

James Hurley

Publication

Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

Insert

yeast Vps23-UEV(1-160) (STP22 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

C133A

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-FGF10-EGFP

Plasmid

#261286

Purpose

Expresses EGFP-tagged mouse FGF10 in mammalian cells

Depositor

Cliff Tabin

Publication

Yan et al bioRxiv 2026.06.16.732783

Insert

Fibroblast Growth Factor 10 (Fgf10 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPBhyg-Tks5-mNeonGreen

Plasmid

#259702

Purpose

For introducing Tks5-mNeonGreen into the genome using the PiggyBac transposon system.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

Tks5 (SH3PXD2A Canis lupus familiaris (dog))

Tags

mNeonGreen

Expression

Mammalian

Promoter

CAG promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO-1ΔPDZ1

Plasmid

#259703

Purpose

For introducing EGFP-ZO-1ΔPDZ1 into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lacks the PDZ1 domain

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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