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p5E-CMVmin

Plasmid

#80802

Purpose

minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCO603

Plasmid

#139736

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnLuz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Luz

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAV0761

Plasmid

#133494

Purpose

Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ChrV_oxTi365_loxP_myo2_neoR

Plasmid

#194057

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

oxTi365

Expression

Worm

Promoter

None

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

Plasmid

#136543

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCO600

Plasmid

#139734

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnHisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Hisps

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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NTS-PC1

Plasmid

#41574

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7 and Myc/Myc/His

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pTRANS_223

Plasmid

#91117

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2C

Plasmid

#202730

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2C (PIP4K2C Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMC-1-ZmAXIG1-6

Plasmid

#197711

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmAXIG1 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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PSuperPuroRetro-1660(hPot1)

Plasmid

#11135

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Cx43-7

Plasmid

#58009

Purpose

Localization: Gap Junctions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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Pdx1-RFP puro (DM#235)

Plasmid

#15085

Depositor

Douglas Melton

Article

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Tags

RFP

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pT2Aneo-tdTomato-CAAX

Plasmid

#170284

Purpose

Encoding membrane-anchored tdTomato.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

tdTomato-CAAX

Expression

Mammalian

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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