We narrowed to 7,375 results for: ef1a

1. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125691

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

   Plasmid

   #133027

   Purpose

   Expresses EGFP-tagged late endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAB7A (RAB7A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

   Plasmid

   #134858

   Purpose

   Expresses EGFP-tagged early endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Ras-related protein 5A
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

   Plasmid

   #201681

   Purpose

   Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

   Depositor

   Emma Rawlins

   Article

   Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

   Inserts

   SFTPC promoter region (2kb upstream)
   TagRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

   Plasmid

   #162230

   Purpose

   Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaG17 GFP nanobody SynNotch tTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Has Service

    AAV9

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-EF1afull-H2B-mCherry-P2A-Fluc

    Plasmid

    #207253

    Purpose

    Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    H2B-mCherry-P2A-Fluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha_full

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

    Plasmid

    #204821

    Purpose

    Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

    Depositor

    Emma Rawlins

    Article

    He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

    Insert

    An upstream region including human SCGB3A2 promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SCGB3A2 promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid

    #117134

    Purpose

    All-in-one expression vector for Cas9 and gRNA against CD44

    Depositor

    Kosuke Yusa

    Insert

    gCD44 v2 (Cd44 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

    Plasmid

    #197687

    Purpose

    plasmid for expressing the DNMT3bCD

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid

    #155305

    Purpose

    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    

    Use

    Lentiviral

    Tags

    2xNLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid

    #228401

    Purpose

    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    SREBP1 (SREBF1 Human)
    

    Use

    Tags

    mNeonGreen2x

    Expression

    Mammalian

    Mutation

    deleted N terminal activation domain (amino acids…

    Promoter

    EF1a

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177435

    Purpose

    To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

    Depositor

    David Andrews

    Insert

    Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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