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Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)

Plasmid Kit

#1000000175

Purpose

Plasmids and Gateway entry vectors for fluorescent sensors of diverse physiological parameters in cells.

Depositor

Adam Cohen

Application

Gene Expression and Labeling

Vector Type

Lentiviral, Mammalian Expression

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
AAV-Synapsin-mTurquoise2-optop38i5-WPRE

Plasmid

#159972

Purpose

optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optop38i5

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-optop38i3-WPRE

Plasmid

#159971

Purpose

optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optop38i3

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-optoJNKi-WPRE

Plasmid

#159968

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mTurquoise2-optoJNKi-WPRE

Plasmid

#159966

Purpose

optogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mTurquoise2-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-optoJNKi-WPRE

Plasmid

#159967

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Golgin-84-TEV #709#243

Plasmid

#42108

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

Golgin-84 (GOLGA5 Human)

Tags

GFP

Expression

Mammalian

Mutation

inserted ENLYFQG b/w 300 and 301

Promoter

CMV

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159961

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159965

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159964

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159958

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159957

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159960

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159959

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

Plasmid

#159963

Purpose

optoNES construct with modified mTurquoise2-LOV2 RET

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

CMV-IE

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-HA [12CA5] - Chimeric (Recombinant Antibody)

Antibody

#198003-rAb

Purpose

Anti-HA chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.

Depositor

Melina Fan

Recommended Applications

Western Blot

Source Species

Rabbit

Isotype

IgG

Trial Size

Available to purchase

Available Since

March 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits
Anti-HA [12CA5] - Chimeric (Recombinant Antibody)

Antibody

#199805-rAb

Purpose

Anti-HA chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.

Depositor

Melina Fan

Recommended Applications

Western Blot

Source Species

Chicken

Isotype

IgY

Trial Size

Available to purchase

Available Since

April 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
Anti-HA [12CA5] (Recombinant Antibody)

Antibody

#194541-rAb

Purpose

Anti-HA recombinant mouse monoclonal antibody

Depositor

Melina Fan

Recommended Applications

Immunocytochemistry and Western Blot

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Feb. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Str-Ii_SBP-mCherry-Golgin84

Plasmid

#65304

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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