Addgene

We narrowed to 1,958 results for: appa

Showing: 821 - 840 of 1958 results

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pRCA938 - pBA904 Puro-T2A-GFP PLAGL1_g1 CRISPRa guide guide (pRCA360 backbone)

Plasmid

#238189

Purpose

Lentiviral CRISPR guide vector expressing a PLAGL1 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

PLAGL1 sgRNA CRISPRa (PLAGL1 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

Plasmid

#238169

Purpose

Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

CD45 sgRNA CRISPRa (PTPRC Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

Plasmid

#175256

Purpose

SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TRAV29

Plasmid

#123401

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV29 (TRAV29DV5 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-210-0)

Plasmid

#89826

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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FNE2-A72

Plasmid

#44608

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2-A72 (Ets2 Mouse)

Use

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

THR-72 converted to Alanine

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
ER-mEGFP

Plasmid

#182876

Purpose

Localization: Endoplasmic Reticulum

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

ER-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pCR3.1-CREM4

Plasmid

#106459

Purpose

CMV promoter with 4 CRE sites mutated, can be used to create a reporter vector

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

4 CRE sites mutated

Use

Tags

Expression

Mammalian

Mutation

4 CRE sites mutated

Promoter

CMV

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVmyc-ORF73

Plasmid

#21545

Depositor

J Simas

Article

Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

Insert

ORF73 (ORF73 MuHV-4)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-A20

Plasmid

#22141

Depositor

Yihong Ye

Article

Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

Insert

A20 (TNFAIP3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RRM2

Plasmid

#13796

Depositor

Edward Whang

Article

Duxbury et al Biochem Biophys Res Commun. 2007 Mar 2.

Insert

RRM2 (RRM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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pABY-T2

Plasmid

#201765

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T1

Plasmid

#201764

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T3

Plasmid

#201766

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pABY-T4

Plasmid

#201767

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST26-hOPTN

Plasmid

#23050

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Use

Tags

His

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCR3.1-cGK1

Plasmid

#106452

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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BiDi-[mEGFP]mCherry2

Plasmid

#182875

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mCherry2 and mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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