Skip to main content
Addgene
Showing: 821 - 840 of 6530 results

  7. SNAP-LC3 HRD

    Plasmid
    #207545
    Purpose
    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    SNAPtag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207545-plasmid-map-sequence-id-430987

    Enlarge

  8. Halo-LC3B HRD

    Plasmid
    #207539
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    207539-plasmid-map-sequence-id-429514

    Enlarge

  10. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

    Enlarge

  11. lentiMutB3GNT5-blast

    Plasmid
    #208380
    Purpose
    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nt 922 c->g (PAM site inactivation)
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208380-plasmid-map-sequence-id-414094

    Enlarge

  12. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Expression
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. R777-E288 Hs.ROS1-nostop

    Plasmid
    #70572
    Purpose
    Gateway ORF clone of human ROS1 [NM_002944.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROS1 (ROS1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70572-plasmid-map-sequence-id-126021

    Enlarge

  18. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

    Enlarge

  19. R777-E102 Hs.HRAS-nostop

    Plasmid
    #70386
    Purpose
    Gateway ORF clone of human HRAS [NM_005343.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70386-plasmid-map-sequence-id-125835

    Enlarge

Showing: 821 - 840 of 6530 results