Skip to main content
Addgene
Showing: 821 - 840 of 1749 results

  2. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

    Enlarge

  4. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid
    #117720
    Purpose
    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    mAID-nanobody
    IBB-mCherry
    Use
    Tags
    importin-beta bining domain (IBB) and triple HA
    Expression
    Mammalian
    Mutation
    codon optimized mAID for murine expression
    Promoter
    CMV and None, IRES-driven
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117720-plasmid-map-sequence-id-227032

    Enlarge

  5. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid
    #112157
    Purpose
    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.
    Depositor
    Ben Giepmans
    Article
    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261
    Insert
    anti-GFP FLIPPER-body
    Use
    Tags
    His and Thrombin cleavage site
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112157-plasmid-map-sequence-id-213698

    Enlarge

  9. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

    Plasmid
    #179901
    Purpose
    Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    antiGFPnanobody-mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179901-plasmid-map-sequence-id-357220

    Enlarge

  11. pAAV-DYRK1A(K188R)-2A-mCherry

    Plasmid
    #177166
    Purpose
    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Changed Lysine 188 to Arginine
    Promoter
    CMV with beta globin intron
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177166-plasmid-map-sequence-id-349444

    Enlarge

  13. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid
    #115782
    Purpose
    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.
    Depositor
    Fraser Sim
    Article
    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1
    Inserts
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    Use
    Lentiviral
    Tags
    EF1α promoter fused to mCherry and cfos promoter …
    Expression
    Mutation
    Promoter
    SOX10 Multiple Species Conserved enhancer element…
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    115782-plasmid-map-sequence-id-226932

    Enlarge

  14. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

    Plasmid
    #164827
    Purpose
    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch
    Depositor
    Wendell Lim
    Article
    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171
    Inserts
    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    miniCMV and pGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    164827-plasmid-map-sequence-id-335845

    Enlarge

  15. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

    Plasmid
    #164828
    Purpose
    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch
    Depositor
    Wendell Lim
    Article
    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171
    Inserts
    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    miniCMV and pGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    164828-plasmid-map-sequence-id-331207

    Enlarge

  16. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Article
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107939-plasmid-map-sequence-id-207466

    Enlarge

  17. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid
    #41863
    Purpose
    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Insert
    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41863-plasmid-map-sequence-id-57052

    Enlarge

Showing: 821 - 840 of 1749 results