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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,627 results for: otos

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Showing: 821 - 840 of 10627 results

  4. DF-UAS-sspa-GCaMP6f

    Plasmid
    #67569
    Purpose
    Zebrafish expression of a short photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    sspa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Available Since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pGTM_Sumo2-C-8XHis-PLpro

    Plasmid
    #234321
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the C-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234321-plasmid-map-sequence-id-469248

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  13. pGTM_Sumo2-N-StrepII-PLpro

    Plasmid
    #234322
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage a Strep-tag II sequence is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234322-plasmid-map-sequence-id-469910

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  14. pGTM_Sumo_PLpro_C111A

    Plasmid
    #234489
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111A active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234489-plasmid-map-sequence-id-469250

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  15. pGTM_Sumo2-N-8XHis-Plpro

    Plasmid
    #233803
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    orf1ab (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233803-plasmid-map-sequence-id-469494

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Showing: 821 - 840 of 10627 results