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Showing: 821 - 840 of 9778 results

  1. AAV-CMV-scFVtet2_bGHpA

    Plasmid
    #177352
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177352-plasmid-map-sequence-id-353673

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  3. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  4. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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  7. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Article
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    50834-plasmid-map-sequence-id-76270

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  8. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177344
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Tetracycline-dependent promoters
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177344-plasmid-map-sequence-id-353629

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  11. AAV-hSyn-scFVtet2_bGHpA

    Plasmid
    #177353
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    human Synapsine promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177353-plasmid-map-sequence-id-353612

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  12. AAV-TRE-scFVDnmt3L_bGHpA

    Plasmid
    #177356
    Purpose
    AAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    194–415 aa
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177356-plasmid-map-sequence-id-353665

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  13. AAV-TRE-scFVtet2_bGHpA

    Plasmid
    #177358
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177358-plasmid-map-sequence-id-353663

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  14. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

    Plasmid
    #195048
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195048-plasmid-map-sequence-id-386137

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  15. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

    Plasmid
    #195047
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195047-plasmid-map-sequence-id-386131

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  16. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

    Plasmid
    #195046
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195046-plasmid-map-sequence-id-386136

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  17. AAVS1-Hb9-CD14

    Plasmid
    #204344
    Purpose
    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hb9 (Mnx1) 9kb promoter fragment
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    204344-plasmid-map-sequence-id-407654

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  20. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

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Showing: 821 - 840 of 9778 results