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pSPgRNA_BLK_P1

Plasmid

#124455

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P1_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P2

Plasmid

#124456

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P2_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P3

Plasmid

#124457

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P4

Plasmid

#124458

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P4_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_P5

Plasmid

#124459

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_P5_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_BLK_E2-1

Plasmid

#124474

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-1_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-2

Plasmid

#124475

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-2_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_BLK_E2-3

Plasmid

#124476

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_BLK_E2-4

Plasmid

#124477

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-4_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA_BLK_E3-3

Plasmid

#124484

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E3-3_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_withR

Plasmid

#139128

Purpose

express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

add arginines

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge_wR

Plasmid

#139119

Purpose

express hnRNPA2 LC with no charged residues with R added back

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

remove all K, most D

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge