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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROS16

Plasmid

#107930

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROS11

Plasmid

#107925

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETDuet RagA-RagC(S75N)

Plasmid

#99645

Purpose

Bacterial expression of RagA-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

S75N

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-CFP-ras

Plasmid

#87451

Purpose

rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

Depositor

Xiaodong Liu

Publication

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

FRB (MTOR Human)

Tags

CFP and ras fragment

Expression

Mammalian

Mutation

FKBP and rapamycin binding domain only

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Publication

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWPT-/CGA-mEGFP-IRES-mCherry

Plasmid

#49233

Purpose

Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Publication

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Lentiviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

EF1-short

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMHBAD14

Plasmid

#42304

Depositor

Hideo Iwai

Publication

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaE C-intein

Tags

GB1-H6

Expression

Bacterial

Promoter

araBAD

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE hygro MEN1 T344R

Plasmid

#11019

Depositor

Matthew Meyerson

Publication

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 T344R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

T344R

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pTBX1-αRabbit-12xSunTag-sfGFP

Plasmid

#254519

Purpose

For use with CoCUT&Tag method, binds rabbit primary antibody and recruits αSunTag-Tn5

Depositor

Derek Janssens

Publication

Sun et al bioRxiv 2026.04.27.721191

Insert

anti-Rabbit::12xSunTag::sfGFP

Tags

Chitin Binding Domain and Mxe GyrA intein

Expression

Bacterial

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTBX1-αMouse-12xMoonTag-mCherry2

Plasmid

#254520

Purpose

For use with CoCUT&Tag method, binds mouse primary antibody and recruits αMoonTag-Tn5

Depositor

Derek Janssens

Publication

Sun et al bioRxiv 2026.04.27.721191

Insert

anti-Mouse::12xMoonTag::mCherry2

Tags

Chitin Binding Domain and Mxe GyrA intein

Expression

Bacterial

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)

Plasmid

#218777

Purpose

Expresses dCAS9-DNMT3A-DNMT3L fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Publication

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV0067 [15ARE23bp -p(cFos)-fLuc-UBC-rLuc-P2A-GFP]

Plasmid

#246421

Purpose

Firefly luciferase driven by an AHR responsive minimal cFOS promoter and a renilla luciferase and GFP driven by a constitutive UBC promoter flipped with respect to the lentiviral backbone

Depositor

Marco Jost

Publication

Verma et al bioRxiv [Preprint]. 2025 Sep 15:2025.09.

Inserts

Firefly luciferase
Renilla luciferase-P2A-EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

AHR responsive minimal cFOS promoter and UbC

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

Plasmid

#234410

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Publication

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Publication

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

Plasmid

#225697

Purpose

Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Publication

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (WT) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mCherry

Expression

Mammalian

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HiFi-nCas9-NG_P2A-mCherry

Plasmid

#219936

Purpose

Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

HiFi-nCas9(D10A/R691A)

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV303

Plasmid

#204978

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

Depositor

Uffe Mortensen

Publication

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
amdS

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Publication

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

p18

Expression

Mammalian

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)

Plasmid

#189795

Purpose

Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Publication

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Human, Mouse)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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