We narrowed to 9,332 results for: yeast expression

1. pCP-tRNA

   Plasmid

   #133812

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
   

   Use

   CRISPR

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAT253

   Plasmid

   #40943

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

   Plasmid

   #116885

   Purpose

   FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RVH)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWKS9

   Plasmid

   #139416

   Purpose

   Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

   Depositor

   James Fraser

   Article

   Spencer et al Fungal Genet Biol. 2020 May;138:103365.

   Insert

   mTurquoise2
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

   Plasmid

   #115698

   Purpose

   ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   SSKar2-sfpHluorin-HDEL
   

   Tags

   ER retention signal containing HDEL and ER signal…

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAT222

   Plasmid

   #40942

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGAN230

   Plasmid

   #131122

   Purpose

   reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   SUP35

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Expression

   Yeast

   Available Since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KBB418

    Plasmid

    #185088

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KBB460

    Plasmid

    #185094

    Purpose

    NMA111-GFP wild type under endogenous promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    NMA111-GFP

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJZC522

    Plasmid

    #62279

    Purpose

    MCP-VP64 fusion

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    MCP-VP64
    

    Tags

    VP64

    Expression

    Yeast

    Promoter

    pAdh

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGWKS7

    Plasmid

    #139414

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. THI VP64 pALD4 T-

    Plasmid

    #161481

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Expression

    Yeast

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S3_001_026_MV2

    Plasmid

    #134422

    Purpose

    pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. yHS1

    Plasmid

    #159150

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1
    

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Promoter

    TDH3 and TEF1

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWKS10

    Plasmid

    #139417

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Clover
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge