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KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13 Aphrodite

Plasmid

#13570

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot3

Plasmid

#79250

Purpose

Expresses EGFP-tagged DmNot3 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot3 (Not3 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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L1-D544N

Plasmid

#15499

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-D544N (L1CAM Human)

Expression

Bacterial

Mutation

Aspartic Acid 544 changed to Asparagine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
CaM-RhoA(DP)-Venus

Plasmid

#31994

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

Insert

CaM-RhoA(DP) (RHOA Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L mutation in RhoA, E7S and D65Y in CaM

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pFbx15(-1120/+114)-Luc

Plasmid

#13411

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPN144

Plasmid

#91673

Purpose

Express sgRNA targeting human TBC1D5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TBC1D5 (TBC1D5 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN145

Plasmid

#91674

Purpose

Express sgRNA targeting human TBC1D5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

TBC1D5 (TBC1D5 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-Y16C

Plasmid

#30372

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-Y16C (PTEN Human)

Expression

Mammalian

Mutation

Y16C

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
3156 pBS Bad cDNA

Plasmid

#8752

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Expression

Bacterial

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
L1-S542P

Plasmid

#15489

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-S542P (L1CAM Human)

Expression

Bacterial

Mutation

Serine 542 changed to Proline

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLM032

Plasmid

#134244

Purpose

Retroviral plasmid with H3.1A EGFP insert

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human H3.1A + eGFP/HALO retroviral tagging

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Syn3F31A

Plasmid

#13398

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. F31 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pIS1 IMP-1 short UTR

Plasmid

#21639

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFUS_A3A

Plasmid

#43851

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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