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pEGFP-Syx1

Plasmid

#35706

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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HSD11B1-FLAG

Plasmid

#24096

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

HSD11beta1 (HSD11B1 Human)

Use

Lentiviral

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
CLPIT Cry2PHR-mCh-LRP6c

Plasmid

#42961

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Retroviral

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pSe-Rp1

Plasmid

#111644

Purpose

For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A

Depositor

Qunxin She

Article

Peng et al Nucleic Acids Res. 2015 Jan;43(1):406-17

Type

Empty backbone

Use

Archaeal expression vector

Promoter

araS

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 D303G IRES mRuby3

Plasmid

#170640

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

D303G

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 D365E IRES mRuby3

Plasmid

#170641

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

D365E

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G

Plasmid

#170646

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D365E

Plasmid

#170649

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) I367T

Plasmid

#170652

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Venus

Plasmid

#139366

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Scarlet 10AA Venus

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-APC11

Plasmid

#19900

Depositor

Yue Xiong

Article

Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

Insert

APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin2-L261A

Plasmid

#73671

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Expression

Plant

Promoter

RPS5A

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f-AAV

Plasmid

#182501

Purpose

GFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotor

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

AAV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-150

Plasmid

#64235

Purpose

Expresses miR-150 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR150 (MIR150 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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