We narrowed to 36,680 results for: kan

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pSB223

Plasmid

#164127

Purpose

New measurement standard

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

YFP-PP7

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKL2013

Plasmid

#172182

Purpose

A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

Depositor

Kan Wang

Article

McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

Insert

mCherry

Use

CRISPR

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Tom20-CR

Plasmid

#171461

Purpose

Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3C2G

Plasmid

#171946

Purpose

Expresses PIK3C2G in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

PIK3C2G (PIK3C2G Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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MtPT4-p3

Plasmid

#160003

Purpose

Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Inserts

DsRed (reverse orientation)
DODAα1
CYP76AD1
cDOPA5GT

Expression

Plant

Promoter

Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

Plasmid

#169186

Purpose

To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

Plasmid

#169191

Purpose

To express nsp10-nsp16 fusion protein in E. coli

Depositor

John Diffley

Article

Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

Insert

14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

Plasmid

#169160

Purpose

To express nsp14-nsp10 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab27a

Plasmid

#170861

Purpose

Mammalian expression of His-GFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab3a

Plasmid

#170863

Purpose

Mammalian expression of His-GFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rabphilin

Plasmid

#170865

Purpose

Mammalian expression of His-GFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC6A8_STOP

Plasmid

#161081

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A8 (SLC6A8 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

Plasmid

#169077

Purpose

Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GFP tag, GGlinker, TEV cleavage site. StrepII and…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12a

Plasmid

#164658

Purpose

Bacterial expression of LbCas12a using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCB301TOR

Plasmid

#164798

Purpose

Oomycete expression vector using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

NPTII

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway Cloning

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pQCH

Plasmid

#164160

Purpose

IncQ BHR plasmid

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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