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pAPInt-mScarlet-Hyg

Plasmid

#236492

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Hyg

Plasmid

#236477

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Nat

Plasmid

#236479

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-G418

Plasmid

#236478

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL678

Plasmid

#207511

Purpose

GmUbi3-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmUBI3

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL679

Plasmid

#207512

Purpose

GmM4-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL681

Plasmid

#207514

Purpose

GmM4-SpRY ABE-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-MET25pr

Plasmid

#221100

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-MET25pr

Plasmid

#221103

Purpose

Methionine-repressible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-CUP1pr

Plasmid

#221106

Purpose

Copper-inducible gene expression

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-U6tetO_shFF4

Plasmid

#229207

Purpose

lvl 1 expresion vector

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Pol3 expresion of shFF4

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Promoter

J23107 and J23119

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS::Δpes-10P::AI::gur-3G

Plasmid

#232613

Purpose

Expression of 'GUR-3' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::QF+hGR::SL2::tagBFP

Plasmid

#232607

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR' and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G

Plasmid

#232605

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS::Δpes-10P::AI::prdx-2G

Plasmid

#232614

Purpose

Expression of 'PRDX-2' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

PRDX-2 (prdx-2 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::lite-1G

Plasmid

#232606

Purpose

Pan-neuronal expression of 'LITE-1' using a genomic fragment, regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

LITE-1 (lite-1 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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