We narrowed to 31,248 results for: LIS

1. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197024

   Purpose

   Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized OsCas12f1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA6-C-3XFLAG-h-Lmbr1l

   Plasmid

   #123462

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   Limb development membrane protein 1 like (LMBR1L Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6-N-3XFLAG-Axin1

   Plasmid

   #123463

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   Axin 1 (Axin1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-SARS2-S-FKO (NC-flag)

   Plasmid

   #159363

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Use

   Tags

   Flag and Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. RANLS-LEHD-BNES

   Plasmid

   #60973

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   Caspase9 substrate sequence (LEHD) is after NLS s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. CL20_mEGFP_ZC3H4_POLR2I

   Plasmid

   #205956

   Purpose

   Express mEGFP-tagged fusion protein, ZC3H4_POLR2I from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   ZC3H4_POLR2I (ZC3H4, POLR2I Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-SARS2-S-G614 (NC-flag)

   Plasmid

   #156419

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

   Plasmid

   #73267

   Purpose

   Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Use

   Tags

   His/V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

   Plasmid

   #73268

   Purpose

   [Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

   Depositor

   Matthew Sweet

   Article

   Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

   Insert

   HDAC7 (Hdac7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF1a-nSpCas9-NG

    Plasmid

    #171692

    Purpose

    Expresses nSpCas9-NG in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9-NG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

    Promoter

    EF1a

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-HTR6-SNAPf-TRIP

    Plasmid

    #189617

    Purpose

    Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6-TRIP_alpha
    

    Use

    AAV

    Tags

    alphaTag in the N-term of HTR6, SNAP-tag in the C…

    Expression

    Mutation

    Promoter

    TRE

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. shTCF7L2 # 2

    Plasmid

    #42558

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. iLID-tdTomato-Omp25

    Plasmid

    #214400

    Purpose

    Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

    Depositor

    Liting Duan

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

    Insert

    iLID-tdTomato-Omp25 (Synj2bp Synthetic, Rat)
    

    Use

    Tags

    iLID-TdTomato-Omp25

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCL-PGK-SPdCas9-BFP-DNMT3B

    Plasmid

    #66820

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97312

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Tbg-CES2A-ΔC-S227A

    Plasmid

    #200560

    Purpose

    Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-FH-AGO2-5xA

    Plasmid

    #91979

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-N-HA-Gp78

    Plasmid

    #123591

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL20_mEGFP_RBM39_VMP1

    Plasmid

    #205910

    Purpose

    Express mEGFP-tagged fusion protein, RBM39_VMP1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    RBM39_VMP1 (RBM39, VMP1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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