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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MigR1-Myc-Nterm TRIB2

Plasmid

#131153

Purpose

Retroviral expression of N terminus of mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 1-145

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCOG75-Sem1 Sc

Plasmid

#83233

Purpose

pCDF-His6-MBP-Sem1 Sc

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

SEM 1 (SEM1 Budding Yeast)

Tags

His-mbp

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF12_P2A_Hygro_Barcode

Plasmid

#120498

Purpose

Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A S46A

Plasmid

#69818

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Tags

V5

Expression

Mammalian

Mutation

S46A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes3

Plasmid

#61535

Purpose

Expresses Hes3 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes3 (Hes3 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

Plasmid

#105202

Purpose

Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-ppp1cab TAL1

Plasmid

#41835

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147/213-siResist

Plasmid

#49858

Purpose

Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, Methionine 147, an…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pKlab813-bGlobin_3xF-BRCA1(full-length)-Y1845D

Plasmid

#249024

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845D mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab814-bGlobin_3xF-BRCA1(full-length)-M1775R

Plasmid

#249025

Purpose

This plasmid expresses the full length BRCA1 sequence (M1775R mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

M1775R

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab816-bGlobin_3xF-BRCA1(full-length)-G1788V

Plasmid

#249026

Purpose

This plasmid expresses the full length BRCA1 sequence (G1788V mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

G1788V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab818-bGlobin_3xF-BRCA1(full-length)-A1708E

Plasmid

#249027

Purpose

This plasmid expresses the full length BRCA1 sequence (A1708E mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab819-bGlobin_3xF-BRCA1(full-length)-Y1845F

Plasmid

#249028

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845F mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845F

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab966-pUC19-HAL-BRCA1-BSD-P2A-2xHA-FKBP12-F36V-HAR-BRCA1

Plasmid

#249004

Purpose

This plasmid expresses the degron tag knock-in sequence at the N-terminus of the endogenous BRCA1 locus

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

FKBP12 (FKBP1A Human)

Use

CRISPR

Tags

2xHA

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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