We narrowed to 30,074 results for: des.2

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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4Δ1-19-HaloTag-FRT

Plasmid

#247456

Purpose

Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMVP-SEC24C

Plasmid

#231559

Purpose

pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

SEC24C (SEC24C Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

silent PAM mutations (G67 (GGG>GGA) and A115 (…

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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K365-iLid

Plasmid

#188456

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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K365-micro

Plasmid

#188457

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T

Plasmid

#147590

Purpose

Insect Expression of DmThor-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CG32016-E (Thor Fly)

Expression

Insect

Mutation

DmThor two silent mutations compared to the seque…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T

Plasmid

#147591

Purpose

Insect Expression of DmThor-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmCup_417-1133 (Thor Fly)

Expression

Insect

Mutation

Thor contains two silent mutations A246C and T321…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T

Plasmid

#147592

Purpose

Insect Expression of DmThor-DmeIF4Ga_687-1666

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-DmeIF4Ga_687-1666 (Thor Fly)

Expression

Insect

Mutation

DmThor two silent mutations A246C and T321C compa…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPN459

Plasmid

#137875

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pMAL-PIAL2M-sim1

Plasmid

#67908

Purpose

Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Tags

MBP

Expression

Bacterial

Mutation

E281-A496; VEDL 425-428 AAA

Promoter

P-lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (PPP)

Plasmid

#62067

Purpose

Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A, P27A

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-sfGFP

Plasmid

#145171

Purpose

Mammalian expression plasmid for soluble ACE2-super folder GFP fusion

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

Plasmid

#70085

Purpose

Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

Depositor

Ross MacGillivray, Anne Mason

Article

Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

Insert

mutated human serum transferrin (TF Human)

Tags

Hexa His tag and N-terminal signal peptide, 4 aa …

Expression

Mammalian

Mutation

Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

Promoter

SV40

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-8h

Plasmid

#154101

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)-G610C

Plasmid

#119827

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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