We narrowed to 17,211 results for: jun
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Plasmid#136509PurposeMammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)DepositorAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only
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pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseGateway CloningTagsmCherry superfolder GFPAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-rtTAc
Plasmid#172121PurposeConstitutive expression of InteinC-rtTACDepositorInsertInteinC-rtTAc
UseAAVPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo
Plasmid#162740PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneExpressionMammalianPromoterCHO Elongation factor 1AAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-TUBG2
Plasmid#171966PurposeExpression in cell lines of the TUBG2 geneDepositorInsertTUBG2 (TUBG2 Human)
ExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GLN, Linear-3, PolyU(1)
Plasmid#166984PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1)
ExpressionMammalianAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire
Plasmid#149690PurposeFor packaging and expression of Chronos-mT-Sapphire in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HRV3C-AcrIF9
Plasmid#141442PurposeExpression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.DepositorInsertAcrIF9
TagsHRV3C cleavage site and Maltose Binding ProteinExpressionBacterialPromoterptacAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-ugpBp-GFPuv
Plasmid#139078PurposeLow phosphate inducible promoter ugpBp for GFP expressionDepositorInsertugpBp-GFPuv
ExpressionBacterialAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SYFP2-CTNNB1
Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
API5-CRISPR
Plasmid#157664PurposeExpresses Cas9 and sgRNA targeting API5DepositorAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
ExpressionPlantPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
ExpressionPlantPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
ExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5xtetO (tet3) Gene Desert
Plasmid#131339PurposegRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.DepositorInsertDesert-gRNA
UseCRISPRExpressionMammalianAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-1
Plasmid#131328PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-2DepositorInsertJARID2-KO-gRNA-1
UseCRISPRExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
JARID2-KO-gRNA-2
Plasmid#131327PurposegRNA to knockout JARID2. Use with JARID2-KO-gRNA-1DepositorInsertJARID2-KO-gRNA-2
UseCRISPRExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only