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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 91,244 results for: cloning

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Showing: 8481 - 8500 of 91244 results

  6. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    171625-plasmid-map-sequence-id-335505

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  9. pKM7

    Plasmid
    #154164
    Purpose
    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Publication
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    mCherry
    Expression
    Insect
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154164-plasmid-map-sequence-id-309401

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  10. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

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  13. nCas9-PPE

    Plasmid
    #140445
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140445-plasmid-map-sequence-id-296171

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  19. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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  20. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Publication
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Tags
    mCherry
    Expression
    Yeast
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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Showing: 8481 - 8500 of 91244 results