We narrowed to 51,901 results for: ANG

1. pKm-HGBE

   Plasmid

   #170334

   Purpose

   a modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized GBEs
   

   Use

   CRISPR

   Mutation

   Cas9n-D10A

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKm-HABE

   Plasmid

   #170332

   Purpose

   a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized ABEs
   

   Use

   CRISPR

   Mutation

   Cas9n-D10A

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBad-PhoCl2c

   Plasmid

   #164035

   Purpose

   Improved PhoCl variant with enhanced dissociation efficiency

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2c
   

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Promoter

   araBad promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Promoter

   pRSV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas12i2-T2A-EGFP

   Plasmid

   #188270

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-PhoCl2f

   Plasmid

   #164036

   Purpose

   Improved PhoCl variant with faster dissociation rate

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2f
   

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Promoter

   araBad promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-NES-PhoCl2f-mCherry

   Plasmid

   #164048

   Purpose

   Optogenetic control of protein localization by PhoCl2f

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2f-mCherry
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PNCA-GFP-dYY1

    Plasmid

    #67791

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

    Insert

    MMLV-LTR-GFP
    

    Mutation

    Deletion of LTR U3 YY1 binding domain

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

12. ePE6d

    Plasmid

    #229029

    Purpose

    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

    Depositor

    Li Juan

    Article

    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcNDA-NES-PhoCl2c-mCherry

    Plasmid

    #164037

    Purpose

    Optogenetic control of protein localization by PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2c-mCherry
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hUbc13

    Plasmid

    #51131

    Purpose

    human ubc13, E2 for synthesis k63 linked ubiquitin chain

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    ubc13 (UBE2N Human)
    

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUS_B2

    Plasmid

    #31019

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15bHis6Tnp

    Plasmid

    #79807

    Purpose

    Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

    Depositor

    Keji Zhao

    Article

    Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

    Insert

    His6Tnp
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7-LacO-LgBiT

    Plasmid

    #216779

    Purpose

    Expresses LgBiT in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    LgBiT
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2-Tet1-CD

    Plasmid

    #83341

    Purpose

    To introduce MS2 coat protein fused Tet1-CD

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    MS2-Tet1-CD
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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