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pPGK-T7/2-CD44v3 (fl)

Plasmid

#137814

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length and N280D in the V3 region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg30-PDGFR_EF1a_mCherry

Plasmid

#186309

Purpose

Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg30-PDGFR
mCherry

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-2A-eGFP(W58X) pcDNA3.1(-)

Plasmid

#172229

Purpose

Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

mCherry-2A-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104140

Purpose

Expresses TREAT-5'TOP reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-Citroff1

Plasmid

#134301

Purpose

Bacterial expression of inverse-response citrate sensor with high binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB416

Plasmid

#74810

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB416

Plasmid

#74810

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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NUDT1

Plasmid

#74660

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT1 (NUDT1 Human)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
NUDT1

Plasmid

#74660

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT1 (NUDT1 Human)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pBbB7k-GFP

Plasmid

#35360

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbB7k-GFP

Plasmid

#35360

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbE7k-RFP

Plasmid

#35315

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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