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pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

Plasmid

#105032

Purpose

Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

gRNA targeting Hgs (Hgs Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

Plasmid

#119318

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C41A, C46A, C840A

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-dTPR

Plasmid

#114048

Purpose

Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-dTPR (TTK Human)

Expression

Mammalian

Mutation

delta TPR (deletion of TPR domain 63-191)

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d60

Plasmid

#114046

Purpose

Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d60 (TTK Human)

Expression

Mammalian

Mutation

delta 60 (deletion of NTE domain)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCOG40-HRS (257-276) Hs

Plasmid

#83236

Purpose

pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

HRS (257-276) Hs (HGS Human)

Expression

Bacterial

Mutation

258-277

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001487098)

Plasmid

#78031

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001148014)

Plasmid

#78028

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001146579)

Plasmid

#78029

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001148653)

Plasmid

#78030

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001146806)

Plasmid

#78001

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001145144)

Plasmid

#78002

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001144933)

Plasmid

#78003

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001487148)

Plasmid

#78004

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001146460)

Plasmid

#75986

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001148530)

Plasmid

#75984

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001147776)

Plasmid

#75985

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5-L518A/Y619E-FRAG

Plasmid

#68874

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa, L518A/Y619E

Promoter

tac

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
NC11 pCDNA3.1 VENUS WTX K292N

Plasmid

#36966

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX K292N (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

K292N

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
NC8 pCDNA3.1 VENUS WTX S286D

Plasmid

#36963

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286D (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286D

Promoter

CMV

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
NC7 pCDNA3.1 VENUS WTX S286A

Plasmid

#36962

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX S286A (AMER1 Human)

Tags

Venus

Expression

Mammalian

Mutation

S286A

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only