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Showing: 1261 - 1290 of 1961 results

  5. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Anne Ephrussi
    Article
    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    71244-plasmid-map-sequence-id-127267

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  6. pRSET-iGluSnFR4s

    Plasmid
    #234443
    Purpose
    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s
    Use
    Tags
    6xHis-Xpress epi
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234443-plasmid-map-sequence-id-464951

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  11. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid
    #170220
    Purpose
    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CDK1 (CDK1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    T14A, Y15F
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170220-plasmid-map-sequence-id-334386

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  12. mCerulean-proα1(I)

    Plasmid
    #119842
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119842-plasmid-map-sequence-id-233869

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  14. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid
    #154196
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC1-A, sgRNA-BC1-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154196-plasmid-map-sequence-id-308676

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  16. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  17. eGFP-proα1(I)

    Plasmid
    #119843
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119843-plasmid-map-sequence-id-240348

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  19. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  20. eGFP-proα2(I)-L

    Plasmid
    #120160
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120160-plasmid-map-sequence-id-233870

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  22. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120161-plasmid-map-sequence-id-233911

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  23. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid
    #170240
    Purpose
    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    S218D, S222D
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170240-plasmid-map-sequence-id-334554

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  28. mApple-proα1(I)

    Plasmid
    #119844
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119844-plasmid-map-sequence-id-233910

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Showing: 1261 - 1290 of 1961 results