We narrowed to 907 results for: lenticrisper

1. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

   Plasmid

   #181975

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NGFR truncation: aa 11-276

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dCas9-CBP core

   Plasmid

   #179543

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NGFR truncation: aa 11-276

   Promoter

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. dCas9-GCN5 core

   Plasmid

   #179551

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Plasmid

   #154430

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas9-p300 HAT

   Plasmid

   #179544

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid

    #154429

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222501

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid

    #222502

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    TRE3GV and hPGK

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas9-CBP HAT

    Plasmid

    #179547

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgRNA-Cre-GpNLuc-sgmSTING

    Plasmid

    #208386

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgSTING (Sting1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LCV2_PSMB2_sgRNA_1

    Plasmid

    #155092

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMB2_sgRNA_1 (PSMB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid

    #222498

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222499

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid

    #222500

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E and E814…

    Promoter

    EFS

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge