We narrowed to 59,520 results for: plasmid
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Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorPublicationUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pGL3-NFAT luciferase
Plasmid#17870Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissueDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x NFAT binding sequence (IL2 )
UseLuciferaseTagsIL2 promoter and LuciferaseExpressionMammalianPromoterIL-2 promoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-EGFP-WPRE (AAV5)
Viral prep#51502-AAV5PurposeReady-to-use AAV5 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLp6.3-Reverse
Plasmid#79117PurposeLentiviral expression of EglN3-ReverseDepositorInsertcontrol plasmid with reverse EglN3 insert (EGLN3 Human)
UseLentiviralTagsV5ExpressionMammalianPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP8m-WPRE (AAV Retrograde)
Viral prep#162375-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)
Viral prep#131004-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sybody Generation Toolbox
Plasmid kit#1000000160PurposePlasmids to generate synthetic nanobodies against soluble proteins, membrane proteins, and protein complexes.DepositorApplicationGene Expression and LabelingVector typeBacterial ExpressionAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40 (AAV5)
Viral prep#100843-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.Syn.GCaMP6s.WPRE.SV40 (#100843). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PT3.5/CMV-EGFRvIII
Plasmid#20280DepositorAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCXLE-hUL
Plasmid#27080PurposeIntegration-free (episomal) expression of human L-MYC and LIN28DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-Cre
Plasmid#55636PurposeExpresses Cre in Mammalian CellsDepositorHas serviceAAV11 and AAV RetrogradeInsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inse…Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnCasPA-BEC
Plasmid#113349PurposeA cytidine deaminase-mediated base-editing plasmid in Pseudomonas speciesDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV SYN flex PSAM4 GlyR IRES EGFP (AAV5)
Viral prep#119741-AAV5PurposeReady-to-use AAV5 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck-GCaMP5G
ExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by